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However, when a wound is created, these cells migrate as a collective into the empty space. To better study these complex migratory dynamics, confinement assays are used to restrict where the cells can migrate. Here, we use circular confinement assays to assess collective cell migration in an isotropic geometry. Cells are first seeded into stencils with circular holes to make “cellular islands”. Upon removal of the stencil barrier, the cells migrate outward. Previous research has indicated that the initial island size dictates key differences in tissue island expansion and cell migration dynamics. Here, we investigate the role of island size in conjunction with tissue fluidity. To control tissue fluidity, we compare two groups of epithelial cells with distinct biophysical properties. MCF-10A is relatively “solid-like”, with individual cells nearly stationary in crowded conditions. Upon inducible overexpression of endocytic protein, Rab5a, the cells become highly collectively migratory and fluid-like. Comparison of the solid-like and fluid-like cell lines revealed stark differences in their expansion. The solid-like cells expanded at a much slower rate, and the cells within the tissue island bulk were generally stationary. Conversely, the fluid-like cells migrated outward more rapidly, and there appeared to be vortices forming within the bulk, differing from the edges of the island. Furthermore, there were differences in the tissue island edge, with the solid-like tissue having a more tortuous and curved edge, whereas the fluid-like edge was smoother and more unified in its expansion. Overall, this work reveals interesting behaviors between fluid-like and solid-like epithelial cells during isotropic tissue island expansion</p>"},{"label":"Digital Identifier","value":"m2026-brandt"},{"label":"Digital Object Identifier (DOI)","value":"https://doi.org/10.14418/f102f046"},{"label":"Digital Origin","value":["born digital"]},{"label":"Discipline","value":["Biology"]},{"label":"Date","value":"May 6, 2026"},{"label":"EDTF Date","value":"D:00 M:00 Y:2026"},{"label":"File Format","value":["application/pdf"]},{"label":"Genre","value":["masters theses"]},{"label":"Group","value":["Theses & Dissertations"]},{"label":"Held By","value":"Wesleyan University Library, Wesleyan University, Middletown, CT, USA"},{"label":"Language","value":["English"]},{"label":"Repository","value":["University Archives"]},{"label":"Rights","value":["In Copyright"]},{"label":"Thesis Advisor(s)","value":["Mitchel, Jennifer"]},{"label":"Type","value":["Text"]}],"description":"Investigating the expansion 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