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In this study circular DNA scaffolds of varying sizes have been designed. Curvature in the DNA ring is induced by the bacterial DNA binding protein Integration Host Factor (IHF). It is a heterodimer with an alpha helical body and two protruding beta sheet arms. These arms interact within the minor groove of DNA, and proline residues within the arms stabilize the DNA bending. Molecular dynamic simulations were run to determine the mechanics and stability of the DNA ring itself, as well as the mechanics and stability of IHF protein binding. Wet lab experiments are also being conducted to create the physical construct. Applications for this scaffold include its use in cryo-electron microscopy (CryoEM), a new molecular imaging technique that enables researchers to determine the structure of biomolecules. CryoEM requires biomolecules to be at least 200 kDa and maintain an axis of symmetry in order to obtain an accurate image of the structure. Thus, attaching biomolecules less than 200 kDa will enable them to be readily visualized by CryoEM. Additional functionality of this scaffold includes the facilitation and subsequent imaging of weak binding interactions.</p>_x000D_"},{"label":"Digital Identifier","value":"m2020-s485"},{"label":"Thesis Advisor(s)","value":["Thayer, Kelly"]},{"label":"Digital Object Identifier (DOI)","value":"https://doi.org/10.14418/wes01.2.267"},{"label":"Discipline","value":["Molecular Biology and Biochemistry"]},{"label":"Date","value":"May 6, 2020"},{"label":"EDTF Date","value":"D:00 M:00 Y:2020"},{"label":"Extent","value":"83 leaves : illustrations, charts ; 29 cm"},{"label":"File Format","value":["application/pdf"]},{"label":"Genre","value":["masters theses"]},{"label":"Digital Origin","value":["born digital"]},{"label":"Type","value":["Text"]}],"description":"Computational studies and physical construction of Cyclized DNA for a Cryo-EM 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