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In addition, transcription is dependent on the environment since an organism’s requirements are largely dependent on the absence or presence of external cues. Therefore, where, when, and how much of a gene is transcribed is dependent on a dynamic network composed of numerous elements. This thesis begins to investigate the changes in a TF’s GRN that occur when we remove S. cerevisiae from the unstressed log-phase growth environment into a stressful environment. By using the Tet-Off system to titrate the expression of Rap1, we generated a series of Rap1 expression levels and examined differential gene expression and quantification of target genes in response to Rap1 depletion in different environments. These environments include glucose depletion and heat shock. This work provides a basis for quantifying the GRFs of Rap1 and target genes with the goal of using these findings to more accurately represent the yeast GRN.</p>"},{"label":"Digital Identifier","value":"m2021-s211"},{"label":"Thesis Advisor(s)","value":["Coolon, Joseph"]},{"label":"Digital Object Identifier (DOI)","value":"https://doi.org/10.14418/wes01.2.313"},{"label":"Discipline","value":["Biology"]},{"label":"Date","value":"May 12, 2021"},{"label":"EDTF Date","value":"D:00 M:00 Y:2021"},{"label":"Extent","value":"90 pages : illustrations ; 28 cm"},{"label":"File Format","value":["application/pdf"]},{"label":"Genre","value":["masters theses"]},{"label":"Digital Origin","value":["born digital"]},{"label":"Type","value":["Text"]}],"description":"Investigating the context dependent regulation of Rap1 and its downstream target genes in Saccharomyces cerevisiae using 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