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This research demonstrated that HU binds the central region of the 4WJ with nanomolar affinity and prefers the stacked form of the 4WJ with two HU dimers binding one junction. Given the similarities in structure and function of HU and IHF, we elected to study the binding affinity of IHF to the 4WJ. We have compared the IHF binding affinity for 4WJ DNA relative to that of duplex DNA with and without a consensus sequence. We determined that IHF has a 1:1 binding stoichiometry to the 4WJ. All binding assays established that IHF binds to the 4WJ lacking a consensus sequence with 2 nM KD. Moreover, anisotropy binding measurements demonstrated that IHF binds to the 4WJ with similar affinity as to H1 duplex DNA.</p>"},{"label":"Degree","value":["Bachelor of Arts with Departmental Honors"]},{"label":"Administrative Note","value":"2010<br>Old URL: https://wesscholar.wesleyan.edu/etd_hon_theses/549<br>Drupal Node ID: 22549<br>author email: obuzovetsky@wesleyan.edu<br>old filename: buzovetsky_olga_2010_160_549<br>PID: ir:1190"},{"label":"Name(s)","value":["Buzovetsky, Olga (creator)"]},{"label":"Discipline","value":["Molecular Biology and Biochemistry"]},{"label":"Type","value":["Text"]}],"description":"Binding parameters of integration host factor to four-way Holliday junction 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