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Administrative Notes: Old URL: https://wesscholar.wesleyan.edu/etd_mas_theses/243
Author Contact: spark03@wesleyan.edu
Local Identifier: 243_park_s_thesis
PID: ir:2592"},{"label":"Degree","value":["Master of Arts"]},{"label":"Description","value":"

Computationally designed mini-proteins are useful as these proteins can be designed to be thermally and chemically stable. Due to their stability, mini-proteins can be used as small-molecule drugs; drug transportation can be done easily as the miniproteins do not have to be carefully stored and will still have high activity. Furthermore, binding assays have shown that mini-proteins can be designed to have high affinity binding to their targets. The flexibility or rigidity of the mini-protein structure influences the stability of the protein as well, and the more rigid the mini-protein is, the more effective it is as a small-molecule drug. This thesis will focus on the molecular motion of these mini-proteins, which will be studied through Nuclear Magnetic Resonance (NMR) Spectroscopy.

T, T, and NOE experiments are conducted in order to obtain R, R, and η values. With the obtained values, R2 values can be calculated, and all of these values will be utilized in a program called ModelFree to obtain Lipari-Szabo order parameters. For the protein EHEE_rd2_0005, it is found that this protein is heterogeneous and is not a static structure, indicating that this protein is fairly rigid but has certain regions in the protein, such as the C-terminus, loops, and regions in the β-strands within the protein that are more flexible, or experience more motion. Circular Dichroism (CD) Spectroscopy will also be performed to try to obtain a melting temperature of the miniproteins of interest.

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